Western blot analysis of Alpha SNAP using a Alpha SNAP monoclonal antibody (Product #MA5-36194). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human placenta tissue lysate, Lane 2: Mouse testis tissue lysate, Lane 3: SH-SY5Y cell lysate